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pHoY108_pAC8_hsDCAF5_1-477
(Plasmid #249181)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249181 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAC8
  • Vector type
    Insect Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DCAF5(1-477)
  • Alt name
    DDB1- and CUL4-associated factor 5
  • Alt name
    BCRG2, KIAA1824, WDR22
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1566
  • Mutation
    Truncation 1-477
  • Entrez Gene
    DCAF5 (a.k.a. BCRG2, BCRP2, D14S1461E, WDR22)
  • Tag / Fusion Protein
    • Strep_BirA_short-linker-TEV (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GATAACCATCTCGCAAATAAATAAGTATTTTACTG
  • 3′ sequencing primer AATTGTCTGTAAATCAACAACGCACAGAATC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHoY108_pAC8_hsDCAF5_1-477 was a gift from Eric Fischer (Addgene plasmid # 249181 ; http://n2t.net/addgene:249181 ; RRID:Addgene_249181)

  • For your References section:

    Targeting DCAF5 suppresses SMARCB1-mutant cancer by stabilizing SWI/SNF. Radko-Juettner S, Yue H, Myers JA, Carter RD, Robertson AN, Mittal P, Zhu Z, Hansen BS, Donovan KA, Hunkeler M, Rosikiewicz W, Wu Z, McReynolds MG, Roy Burman SS, Schmoker AM, Mageed N, Brown SA, Mobley RJ, Partridge JF, Stewart EA, Pruett-Miller SM, Nabet B, Peng J, Gray NS, Fischer ES, Roberts CWM. Nature. 2024 Apr;628(8007):442-449. doi: 10.1038/s41586-024-07250-1. Epub 2024 Mar 27. 10.1038/s41586-024-07250-1 PubMed 38538798
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