pComb3X-goldengatefriendly
(Plasmid
#249256)
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Purpose(Empty Backbone) pComb3X plasmid engineered for golden gate insertion of antibodies
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249256 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBluescript
- Backbone size (bp) 4356
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Vector typeGolden Gate vector
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe pComb3 plasmid was originally created by Carlos Barbas, Addgene plasmid #63888.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pComb3X-goldengatefriendly was a gift from Sarel Fleishman (Addgene plasmid # 249256 ; http://n2t.net/addgene:249256 ; RRID:Addgene_249256) -
For your References section:
Structure-based design of antibody repertoires with drug-like properties. Tennenhouse A, Wilen R, Gaiduk S, Borni S, Yardeni EH, Goldsmith M, Albeck S, Unger T, Diskin R, Fleishman SJ. bioRxiv 2025.12.10.693474 10.64898/2025.12.10.693474
