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pACYC-PJ23100-dpGFP
(Plasmid #249257)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249257 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACYC
  • Backbone size w/o insert (bp) 3853
  • Total vector size (bp) 4567
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    dpGFP
  • Alt name
    PIII-4+S65T
  • Species
    Aequorea victoria
  • Insert Size (bp)
    714
  • Promoter PJ23100

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer AAGAGGAGAAAGGATCCATGAGCAAGAGCAACGAGCTGT
  • 3′ sequencing primer CTCGAGAAGCTTTCTAGATTATCACTTGTACAGCTCGTCCATGCC
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Gene encoded avGFP was from Biswas et al. Nat. Methods. 2021;18: 389-396.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pACYC-PJ23100-dpGFP was a gift from Xiaofeng Yang (Addgene plasmid # 249257 ; http://n2t.net/addgene:249257 ; RRID:Addgene_249257)

  • For your References section:

    An iterative deep learning-guided algorithm for directed protein evolution. Li X, Wang Q, Li J, Tang F, Yang X, Lin Z. iScience. 2025 Aug 7;28(9):113324. doi: 10.1016/j.isci.2025.113324. eCollection 2025 Sep 19. 10.1016/j.isci.2025.113324 PubMed 40927680
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