pIGc_GBP*_ALFAtag
(Plasmid #249263)

• Purpose: Expression in bacteria of GBP* (modified GFP binding protein) with single lysine group and ALFA tag to perform dual light-electron microscopy on (G)FP-tagged proteins
• Depositing Lab: Matthieu Sainlos
• Publication: Ducrot et al bioRxiv 2025.10.08.681152

Backbone

• Vector backbone: pIGc
• Backbone size w/o insert (bp): 5280
• Total vector size (bp): 5753
• Modifications to backbone: Homemade vector derived from pET-24-a+, engineered to incorporate the gene of interest immediately after the start codon in frame with a C-terminal decahistidine tag .
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GBP*
• Alt name: modified GFP binding protein
• Species: Synthetic
• Insert Size (bp): 471
• Promoter: T7
• Tags / Fusion Proteins:
  • ALFA tag (C terminal on insert)
  • His tag (C terminal on insert)
  • Single lysine for NHS-ester coupling (C terminal on insert)

Cloning Information

• Cloning method: Other
• 5′ sequencing primer: T7 promoter, Forward primer
• 3′ sequencing primer: T7 terminator, reverse primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The expressed gene was synthesized and the sequence is derived (i.e., contains mutations) from the PDB structure sequence 3K1K.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.1101/2025.10.08.681152 for bioRxiv preprint.