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pLV-mCherry:T2A:Bsd-EF1A-HA3xGShGLI1
(Plasmid #249270)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249270 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pLV
  • Vector type
    Lentiviral
  • Selectable markers
    Blasticidin ; mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GLI1
  • Species
    H. sapiens (human)
  • GenBank ID
    NM_001160045
  • Entrez Gene
    GLI1 (a.k.a. GLI, PAPA8, PPD1)
  • Promoter EF-1
  • Tag / Fusion Protein
    • HA + 3xGS linker (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLV-mCherry:T2A:Bsd-EF1A-HA3xGShGLI1 was a gift from Ramiro Iglesias-Bartolome (Addgene plasmid # 249270 ; http://n2t.net/addgene:249270 ; RRID:Addgene_249270)

  • For your References section:

    Dissection of Galphas and Hedgehog Signaling Crosstalk Reveals Therapeutic Opportunities to Target Hedgehog-Dependent Tumors. Krantz S, Bell BA, Lund K, Salinas Parra N, Ng Y, De Oliveira Rosa N, Mukhopadhyay S, St Croix B, Sarin KY, Weigert R, Raimondi F, Iglesias-Bartolome R. Cancer Res. 2025 Dec 29. doi: 10.1158/0008-5472.CAN-25-1109. 10.1158/0008-5472.CAN-25-1109 PubMed 41460725
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