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CHIKV eGFP-Zeo replicon
(Plasmid #249289)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 249289 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 2283
  • Total vector size (bp) 11546
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    CHIKV LR2006-OPY1 replicon harboring nonstructural genes (nsp1-nsp4) and eGFP-Zeocin ORF in place of structural genes
  • Species
    Chikungunyavirus virus
  • Insert Size (bp)
    9229
  • Mutation
    structural polyprotein open reading frame deleted and replaced with eGFP-Zeocin reporter selection cassette
  • GenBank ID
    DQ443544.2 Chikungunya virus strain LR2006_OPY1, complete genome
  • Promoter SP6

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid is a modified version of the CHIKV replicons described in Remenyi, R., Roberts, G.C., Zothner, C. et al. SNAP-tagged Chikungunya Virus Replicons Improve Visualisation of Non-Structural Protein 3 by Fluorescence Microscopy. Sci Rep 7, 5682 (2017). https://doi.org/10.1038/s41598-017-05820-0.

Please visit https://doi.org/10.1101/2025.01.09.632058 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CHIKV eGFP-Zeo replicon was a gift from Amy Kistler (Addgene plasmid # 249289 ; http://n2t.net/addgene:249289 ; RRID:Addgene_249289)
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