pNORg14-mScarlet-I
(Plasmid
#249343)
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PurposeMediterraneibacter gnavus - Escherichia coli shuttle vector with mScarlet-I under the control of the cumate-inducible promoter and theophylline-inducible riboswitch
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249343 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepNORg10
- Backbone size w/o insert (bp) 6603
- Total vector size (bp) 8384
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Vector typeE. coli - R. gnavus shuttle vector
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert namecymR
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Insert Size (bp)612
Gene/Insert 2
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Gene/Insert namefdx promoter
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Insert Size (bp)200
Gene/Insert 3
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Gene/Insert nameveg promoter
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Insert Size (bp)134
Gene/Insert 4
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Gene/Insert nameCuO
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Insert Size (bp)28
Gene/Insert 5
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Gene/Insert nametheophylline riboswitch
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Insert Size (bp)36
Gene/Insert 6
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Gene/Insert namemScarlet-I
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Insert Size (bp)696
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.08.12.669022 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNORg14-mScarlet-I was a gift from Nozomu Obana (Addgene plasmid # 249343 ; http://n2t.net/addgene:249343 ; RRID:Addgene_249343) -
For your References section:
A genetic toolkit for the human gut bacterium Mediterraneibacter gnavus identifies capsular polysaccharides as a competitive colonization factor. Obana N, Nakato G, Nomura N, Fukuda S. Nat Commun. 2026 Mar 12. doi: 10.1038/s41467-026-69022-x. 10.1038/s41467-026-69022-x PubMed 41820342