pAAV_Ef1a_DIO_hTyrHA_superslowControl
(Plasmid
#249346)
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PurposeControl hTyr (D383N/A206T) — human tyrosinase carrying D383N and A206T substitutions; catalytic activity super slow; intended as an enzymatically-impaired control.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249346 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepAAV
- Total vector size (bp) 6586
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert namemutated super slow human Tyrosinase
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SpeciesH. sapiens (human)
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Insert Size (bp)1594
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MutationD383N/A206T
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GenBank ID7299
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Entrez GeneTYR (a.k.a. ATN, CMM8, OCA1, OCA1A, OCAIA, SHEP3)
- Promoter EF1a
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Tag
/ Fusion Protein
- HA tag (C terminal on insert)
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer EF1a_for
- 3′ sequencing primer WPRE_rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV_Ef1a_DIO_hTyrHA_superslowControl was a gift from Matthias Prigge (Addgene plasmid # 249346 ; http://n2t.net/addgene:249346 ; RRID:Addgene_249346)
Map uploaded by the depositor.
