pHR-Zeo-CMV-TetO2
(Plasmid
#249349)
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PurposeMammalian (inducible) protein expression
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249349 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepHR-Zeo-CMV-TetO2
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Vector typeLentiviral
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemNeonGreen
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SpeciesBranchiostoma lanceolatum
- Promoter CMV-MIE
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer CGTCGTCGTGCTCGTTTAGTG
- 3′ sequencing primer GAGCAACATAGTTAAGAATACCAGTC
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR-Zeo-CMV-TetO2 was a gift from Jonathan Elegheert (Addgene plasmid # 249349 ; http://n2t.net/addgene:249349 ; RRID:Addgene_249349)