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EBOV Zeo-eGFP minigenome
(Plasmid #249393)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 249393 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC57
  • Backbone manufacturer
    Genscript
  • Backbone size w/o insert (bp) 2942
  • Total vector size (bp) 5391
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zeocin-eGFP fusion gene flanked by EBOV 5'UTR and 3'UTR
  • Alt name
    EBOV Zeo-eGFP minigenome
  • Species
    Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga
  • Insert Size (bp)
    2441
  • GenBank ID
    NC_002549.1 Zaire ebolavirus isolate Ebola virus/H.sapiens-tc/COD/1976/Yambuku-Mayinga, complete genome
  • Promoter T7

Cloning Information

  • Cloning method Gene Synthesis
  • 5′ sequencing primer GTAAAACGACGGCCAGT
  • 3′ sequencing primer ccgtcgactgcagaggcctgc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.01.09.632058 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EBOV Zeo-eGFP minigenome was a gift from Amy Kistler (Addgene plasmid # 249393 ; http://n2t.net/addgene:249393 ; RRID:Addgene_249393)
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