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pET-28a ALDOC-K14R
(Plasmid #249431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249431 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET-28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositing lab recommends using BL21 cells for protein expression.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ALDOC
  • Species
    H. sapiens (human)
  • Mutation
    K14R
  • Entrez Gene
    ALDOC (a.k.a. ALDC)
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-28a ALDOC-K14R was a gift from Sheng-cai Lin (Addgene plasmid # 249431 ; http://n2t.net/addgene:249431 ; RRID:Addgene_249431)

  • For your References section:

    Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination. Sun Y, Zhang WW, Men L, Wu J, Yao L, Huang X, Wu Y, Zhang C, Chen Y, Carling D, Zhang CS, Lin SC. Nat Metab. 2025 Oct 22. doi: 10.1038/s42255-025-01386-8. 10.1038/s42255-025-01386-8 PubMed 41125938
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