pcDNA 3.3 N-MYC AMPKγ1
(Plasmid
#249432)
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PurposeUsed for transfection of cells for expression of N-MYC AMPKγ1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249432 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA 3.3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAMPKγ1
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SpeciesH. sapiens (human)
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Entrez GenePRKAG1 (a.k.a. AMPKG)
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Tag
/ Fusion Protein
- MYC (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA 3.3 N-MYC AMPKγ1 was a gift from Sheng-cai Lin (Addgene plasmid # 249432 ; http://n2t.net/addgene:249432 ; RRID:Addgene_249432) -
For your References section:
Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination. Sun Y, Zhang WW, Men L, Wu J, Yao L, Huang X, Wu Y, Zhang C, Chen Y, Carling D, Zhang CS, Lin SC. Nat Metab. 2025 Oct 22. doi: 10.1038/s42255-025-01386-8. 10.1038/s42255-025-01386-8 PubMed 41125938