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pcDNA 3.3 N-MYC AMPKγ1-D317A
(Plasmid #249433)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249433 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA 3.3
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AMPKγ1
  • Species
    H. sapiens (human)
  • Mutation
    D317A
  • Entrez Gene
    PRKAG1 (a.k.a. AMPKG)
  • Tag / Fusion Protein
    • MYC (N terminal on insert)

Cloning Information

  • Cloning method Ligation Independent Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.3 N-MYC AMPKγ1-D317A was a gift from Sheng-cai Lin (Addgene plasmid # 249433 ; http://n2t.net/addgene:249433 ; RRID:Addgene_249433)

  • For your References section:

    Oligodendrocyte precursor cell-specific blocking of low-glucose-induced activation of AMPK ensures myelination and remyelination. Sun Y, Zhang WW, Men L, Wu J, Yao L, Huang X, Wu Y, Zhang C, Chen Y, Carling D, Zhang CS, Lin SC. Nat Metab. 2025 Oct 22. doi: 10.1038/s42255-025-01386-8. 10.1038/s42255-025-01386-8 PubMed 41125938
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