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pCAG-TSF-ULK1-ADA
(Plasmid #249534)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249534 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ULK1
  • Alt name
    ATG1
  • Alt name
    UNC51
  • Alt name
    ATG1A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3153
  • Mutation
    P433A, V434D and P435A
  • GenBank ID
    NM_003565.4
  • Entrez Gene
    ULK1 (a.k.a. ATG1, ATG1A, UNC51, Unc51.1, hATG1)
  • Promoter CAG
  • Tags / Fusion Proteins
    • strep-tag (C terminal on insert)
    • Flag-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer GTTCGGCTTCTGGCGTGT
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.07.687251 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-TSF-ULK1-ADA was a gift from James Hurley (Addgene plasmid # 249534 ; http://n2t.net/addgene:249534 ; RRID:Addgene_249534)

  • For your References section:

    Reconstitution of multistep recruitment of ULK1 to membranes in autophagy. Duan Y, Lu Y, Paul S, Betz J, Wilhelm L, Cook ASI, Ren X, Adriaenssens E, Martens S, Ganley IG, Hummer G, Hurley JH. bioRxiv [Preprint]. 2025 Nov 9:2025.11.07.687251. doi: 10.1101/2025.11.07.687251. 10.1101/2025.11.07.687251 PubMed 41278708
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