pBMN-HA-ATG13-HF|DD
(Plasmid
#249535)
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PurposeExpressing human full length ATG13 with H214D/F215D mutations
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249535 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBMN
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATG13
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Alt nameKIAA0652
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Alt namePARATARG8
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SpeciesH. sapiens (human)
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Insert Size (bp)1551
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MutationH214D and F215D
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GenBank IDNM_001346311.2
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Entrez GeneATG13 (a.k.a. KIAA0652, PARATARG8)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GCTTGGATACACGCCGC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.11.07.687251 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBMN-HA-ATG13-HF|DD was a gift from James Hurley (Addgene plasmid # 249535 ; http://n2t.net/addgene:249535 ; RRID:Addgene_249535) -
For your References section:
Reconstitution of multistep recruitment of ULK1 to membranes in autophagy. Duan Y, Lu Y, Paul S, Betz J, Wilhelm L, Cook ASI, Ren X, Adriaenssens E, Martens S, Ganley IG, Hummer G, Hurley JH. bioRxiv [Preprint]. 2025 Nov 9:2025.11.07.687251. doi: 10.1101/2025.11.07.687251. 10.1101/2025.11.07.687251 PubMed 41278708