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pCas9-EGFP_SLEEK
(Plasmid #249545)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249545 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    px459
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    5'arm-Exon9-Cas9-EGFP-3'arm
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    6379
  • GenBank ID
  • Promoter U6 promoter
  • Tag / Fusion Protein
    • EGFP

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ggacgaaacaccgatttagactacAAGACTGTGGATGGCCCCTCCGGGAA
  • 3′ sequencing primer gttttagtccccttcgtttttgggACTGGAGCGAGGAGAGGAAAAGGCCT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas9-EGFP_SLEEK was a gift from Joe Zhang (Addgene plasmid # 249545 ; http://n2t.net/addgene:249545 ; RRID:Addgene_249545)
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