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pCX-FLAG-KAT2B
(Plasmid #249547)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249547 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCX
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kat2B
  • Alt name
    p300/CBP-associated factor (P/CAF)
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2520
  • GenBank ID
    U57317
  • Entrez Gene
    KAT2B (a.k.a. CAF, P/CAF, PCAF)
  • Promoter CAG
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer GGTTCGGCTTCTGGCGTGTGACC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCX-FLAG-KAT2B was a gift from Xiang-Jiao Yang (Addgene plasmid # 249547 ; http://n2t.net/addgene:249547 ; RRID:Addgene_249547)

  • For your References section:

    A p300/CBP-associated factor that competes with the adenoviral oncoprotein E1A. Yang XJ, Ogryzko VV, Nishikawa J, Howard BH, Nakatani Y. Nature. 1996 Jul 25. 382(6589):319-24. 10.1038/382319a0 PubMed 8684459
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