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pCAG-MitoRlucN-T2A-RlucCER-IRES-mCherry
(Plasmid #249563)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249563 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAG-IRES-mCherry
  • Backbone size w/o insert (bp) 6108
  • Total vector size (bp) 7439
  • Vector type
    Mammalian Expression, Luciferase, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MitoRlucN-T2A-RlucCER
  • Species
    Synthetic
  • Insert Size (bp)
    1320
  • Promoter Chicken beta-actin promoter with CMV enhancer

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer tgctgtctcatcattttggcaaagcaccATGGCCATCCAGCTGCGGTC
  • 3′ sequencing primer cccctagaagcttctgcagatcatttcatgaacaagccaacgaaca
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-MitoRlucN-T2A-RlucCER-IRES-mCherry was a gift from Ginam Cho & Jeffrey Golden & Youngshin Lim (Addgene plasmid # 249563 ; http://n2t.net/addgene:249563 ; RRID:Addgene_249563)

  • For your References section:

    Split-Luciferase Reassembly Assay to Measure Endoplasmic Reticulum-Mitochondria Contacts in Live Cells. Chen C, Rafael KA, Cho G, Lim Y. J Vis Exp. 2024 Oct 11;(212):10.3791/66862. doi: 10.3791/66862. 10.3791/66862 PubMed 39465944
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