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pX458_GAPDH-sgRNA
(Plasmid #249575)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249575 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    px458
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    GAPDH-sgRNA
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    20
  • Promoter U6 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bbsl (destroyed during cloning)
  • 3′ cloning site Bbsl (destroyed during cloning)
  • 5′ sequencing primer ATGGACTATCATATGCTTACCGTA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX458_GAPDH-sgRNA was a gift from Joe Zhang (Addgene plasmid # 249575 ; http://n2t.net/addgene:249575 ; RRID:Addgene_249575)
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