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pCMV-CRY2hiclu-mCherry
(Plasmid #249577)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249577 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone size w/o insert (bp) 4670
  • Total vector size (bp) 6191
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRY2PHR E490R/DLDN
  • Alt name
    CRY2hiclu
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    1521
  • Mutation
    Mutated VATM to DLDN (residues 504-507) from CRY2high-mCherry (Addgene #104063)
  • GenBank ID
    839529
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please refer to related article for further details about the origin and use of this plasmid. Site-directed mutagenesis was performed over vector made by Bianxiao Cui Lab (Addgene #104063).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-CRY2hiclu-mCherry was a gift from Tom Misteli (Addgene plasmid # 249577 ; http://n2t.net/addgene:249577 ; RRID:Addgene_249577)

  • For your References section:

    OptoLoop: An optogenetic tool to probe the functional role of genome organization. Stortz M, Keikhosravi A, Pegoraro G, Misteli T. bioRxiv 2025.11.06.686574 10.1101/2025.11.06.686574
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