pHAGE-dCas9-mCherry-CRY2
(Plasmid #249579)

• Purpose: Lentiviral vector for stable expression of dCas9-mCherry-CRY2hiclu fusion protein in mammalian cells
• Depositing Lab: Tom Misteli
• Publication: Stortz et al bioRxiv 2025.11.06.686574

Backbone

• Vector backbone: pHAGE-DEST
• Backbone size w/o insert (bp): 6607
• Total vector size (bp): 13064
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: CRY2hiclu
• Alt name: CRY2PHR E490R/DLDN
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1521
• GenBank ID: 839529
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-Forward

Gene/Insert 2

• Gene/Insert name: Sp dCas9
• Species: S. pyogenes
• Insert Size (bp): 4101
• Mutation: Catalytically dead mutant Cas9
• GenBank ID: 69900935
• Promoter: CMV
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: CMV-Forward

Resource Information

• Supplemental Documents:
  • pHAGE-dCas9-mCh-CRY2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please refer to related article for further details about origin and use of this plasmid. CRY2 was cloned into vector made by Thoru Pederson lab (Addgene #64108).