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pHAGE-dCas9-3XGFP-CIBN
(Plasmid #249581)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249581 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pHAGE-DEST
  • Backbone size w/o insert (bp) 6607
  • Total vector size (bp) 13545
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    CIBN
  • Alt name
    cryptochrome-interacting basic-helix-loop-helix 1
  • Alt name
    CIB1
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    507
  • Mutation
    N-terminal portion of CIB1
  • GenBank ID
    829604
  • Entrez Gene
    CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XsfGFP (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-Forward
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    Sp dCas9
  • Species
    S. pyogenes
  • Insert Size (bp)
    4101
  • Mutation
    Catalytically dead mutant Cas9
  • GenBank ID
    69900935
  • Promoter CMV
  • Tag / Fusion Protein
    • 3XsfGFP (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please refer to related article for further details about origin and use of this plasmid. CIBN was cloned into vector made by Thoru Pederson lab (Addgene #64107).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE-dCas9-3XGFP-CIBN was a gift from Tom Misteli (Addgene plasmid # 249581 ; http://n2t.net/addgene:249581 ; RRID:Addgene_249581)

  • For your References section:

    OptoLoop: An optogenetic tool to probe the functional role of genome organization. Stortz M, Keikhosravi A, Pegoraro G, Misteli T. bioRxiv 2025.11.06.686574 10.1101/2025.11.06.686574
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