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WEwus
(Plasmid #249675)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249675 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pTRANS_231
  • Backbone manufacturer
    Cambia
  • Backbone size w/o insert (bp) 11947
  • Total vector size (bp) 219819
  • Vector type
    Plant Expression
  • Selectable markers
    Basta

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    EPI300
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WIND1, ESR1, ZmWUS, RUBY, gRNAs
  • gRNA/shRNA sequence
    TTGGTAGTAGCGACTCCATG
  • Species
    Arabidopsis, Maize, Nicotiana benthamiana

Cloning Information

  • Cloning method Golden Gate
  • 5′ sequencing primer Unknown (Unknown if destroyed)
  • 3′ sequencing primer Unknown (Unknown if destroyed)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    WEwus was a gift from Gunvant Patil (Addgene plasmid # 249675 ; http://n2t.net/addgene:249675 ; RRID:Addgene_249675)

  • For your References section:

    A synthetic transcription cascade enables direct in planta shoot regeneration for transgenesis and gene editing in multiple plants. Kshetry AO, Ghose K, Alok A, Devkar V, Raman V, Stupar RM, Herrera-Estrella L, Zhang F, Patil GB. Mol Plant. 2025 Nov 6:S1674-2052(25)00322-3. doi: 10.1016/j.molp.2025.09.017. 10.1016/j.molp.2025.09.017 PubMed 41202820
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