A synthetic transcription cascade enables direct in planta shoot regeneration for transgenesis and gene editing in multiple plants.
Kshetry AO, Ghose K, Alok A, Devkar V, Raman V, Stupar RM, Herrera-Estrella L, Zhang F, Patil GB
Mol Plant. 2025 Nov 6:S1674-2052(25)00322-3. doi: 10.1016/j.molp.2025.09.017.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 249641 | WEipt1 | BeYDV replicon with WIND1, ESR1-ipt cascade, sgRNAs targeting N. benthamiana PDS. RUBY as reporter. |
| 249642 | WEipt2 | BeYDV replicon with WIND1, ESR1-ipt cascade, Cas9 and gRNAs targeting soybean PDS, RUBY as a reporter |
| 249649 | WINDruby | BeYDV replicon with WIND1 and RUBY cascade |
| 249650 | WEruby | BeYDV replicon with WIND1, ESR1-RUBY cascade |
| 249675 | WEwus | BeYDV replicon with WIND1, ESR1-ZmWUS cascade, sgRNA targeting N. benthamiana PDS. RUBY as reporter. |
| 249677 | WEbbm | BeYDV replicon with WIND1, ESR1-GmBBM cascade, sgRNA targeting N. benthamiana PDS. RUBY as reporter. |
| 249678 | ESRruby | BeYDV replicon with ESR1-RUBY cascade. |