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pCAG-GCaMP6f
(Plasmid #249680)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249680 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCAGGS
  • Backbone size w/o insert (bp) 4792
  • Total vector size (bp) 6145
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GCaMP6f
  • Species
    Synthetic
  • Insert Size (bp)
    1353
  • Promoter CAG

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.09.13.612584 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-GCaMP6f was a gift from Takeshi Imai (Addgene plasmid # 249680 ; http://n2t.net/addgene:249680 ; RRID:Addgene_249680)

  • For your References section:

    Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Nat Methods. 2026 Mar 12. doi: 10.1038/s41592-026-03023-y. 10.1038/s41592-026-03023-y PubMed 41820664
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