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pCMV6-TOP3B (WT)-3xFLAG
(Plasmid #249681)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249681 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pCMV6
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human TOP3B
  • Species
    H. sapiens (human)
  • Entrez Gene
    TOP3B (a.k.a. TOP3B1)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFLAG (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV6-TOP3B (WT)-3xFLAG was a gift from Michael Kearse (Addgene plasmid # 249681 ; http://n2t.net/addgene:249681 ; RRID:Addgene_249681)

  • For your References section:

    An autism spectrum disorder mutation in Topoisomerase 3beta causes accumulation of covalent mRNA intermediates by disrupting metal binding within the zinc finger domain. Warrick JE, Attili D, van Eeuwen T, Pastore B, Hoffmann-Weitsman SE, Forsyth NC, Tang W, Barmada SJ, Kearse MG. Nucleic Acids Res. 2025 Oct 28;53(20):gkaf1138. doi: 10.1093/nar/gkaf1138. 10.1093/nar/gkaf1138 PubMed 41189056
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