pGY150
(Plasmid
#249722)
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Purpose(Empty Backbone) pGY150 is a CRISPR adenine base editing vector with the nickase variant of SpCas9-NG (nCas9-NG) and an sgRNA cassette, enabling targeted A-to-G conversions at flexible NG PAM sites in filamentous fung
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 249722 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepGY18
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Vector typeCRISPR, Synthetic Biology
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-7603375/v1 for preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGY150 was a gift from Kyle Pomraning (Addgene plasmid # 249722 ; http://n2t.net/addgene:249722 ; RRID:Addgene_249722) -
For your References section:
Expanding the genetic toolkit: adenine and cytosine base editors for gene disruption in Aspergillus niger. Yuan G, Deng S, Dai Z, Hofstad BA, Pomraning KR. Microb Cell Fact (2026). doi: 10.1186/s12934-026-02979-y 10.1186/s12934-026-02979-y
Map uploaded by the depositor.
