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pcDNA3.1-GSDMD-Flag WT
(Plasmid #249726)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249726 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gasdermin D
  • Species
    H. sapiens (human)
  • Entrez Gene
    GSDMD (a.k.a. DF5L, DFNA5L, FKSG10, GSDMDC1)
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-GSDMD-Flag WT was a gift from Hao Wu (Addgene plasmid # 249726 ; http://n2t.net/addgene:249726 ; RRID:Addgene_249726)

  • For your References section:

    ROS-dependent S-palmitoylation activates cleaved and intact gasdermin D. Du G, Healy LB, David L, Walker C, El-Baba TJ, Lutomski CA, Goh B, Gu B, Pi X, Devant P, Fontana P, Dong Y, Ma X, Miao R, Balasubramanian A, Puthenveetil R, Banerjee A, Luo HR, Kagan JC, Oh SF, Robinson CV, Lieberman J, Wu H. Nature. 2024 Jun;630(8016):437-446. doi: 10.1038/s41586-024-07373-5. Epub 2024 Apr 10. 10.1038/s41586-024-07373-5 PubMed 38599239
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