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RecA-Ts
(Plasmid #249731)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249731 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Ts vector
  • Backbone size w/o insert (bp) 9700
  • Total vector size (bp) 11000
  • Modifications to backbone
    yes
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    RecA
  • Species
    bacteria
  • Insert Size (bp)
    1300
  • Promoter RecA promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI/HindIII/BamHI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer NA
  • 3′ sequencing primer NA
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RecA-Ts was a gift from Nathaniel Heintz (Addgene plasmid # 249731 ; http://n2t.net/addgene:249731 ; RRID:Addgene_249731)

  • For your References section:

    Rapid bacterial artificial chromosome modification for large-scale mouse transgenesis. Gong S, Kus L, Heintz N. Nat Protoc. 2010 Sep;5(10):1678-96. doi: 10.1038/nprot.2010.131. Epub 2010 Sep 30. 10.1038/nprot.2010.131 PubMed 20885380
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