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HRPEadh-pAG305
(Plasmid #249734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249734 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pAG305
  • Backbone manufacturer
    Susan Lindquist (Addgene plasmid # 14138)
  • Backbone size (bp) 7847
  • Modifications to backbone
    GPD replaced with a synthetic 5xHRPE-ADHutr sequence
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.01.673502 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HRPEadh-pAG305 was a gift from Francesco Licausi (Addgene plasmid # 249734 ; http://n2t.net/addgene:249734 ; RRID:Addgene_249734)

  • For your References section:

    A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants. Lavilla-Puerta M, He Y, Piccinini L, Di Paco L, Papachristodoulou A, Licausi F, Giuntoli B. bioRxiv 2025.09.01.673502 10.1101/2025.09.01.673502
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