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UbSYRAP_pDONR201
(Plasmid #249816)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249816 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pDONR201
  • Backbone manufacturer
    Thermo Fisher scientific
  • Backbone size w/o insert (bp) 2040
  • Total vector size (bp) 4253
  • Vector type
    Yeast Expression, Plant Expression, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    UBQ-RAP2.12-GAL4STE
  • Alt name
    SYRAP
  • Species
    S. cerevisiae (budding yeast), A. thaliana (mustard weed)
  • Insert Size (bp)
    1992
  • GenBank ID
    NP_175794.1
  • Entrez Gene
    RAP2.12 (a.k.a. AT1G53910, T18A20.14, T18A20_14, related to AP2 12)
  • Tags / Fusion Proteins
    • GAL4-STE12 (C terminal on insert)
    • UBQ (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer CCTGGCAGTTCCCTACTCTCGCGTT
  • 3′ sequencing primer ATGTAACATCAGAGATTTTGAGACA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.09.01.673502 for the bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    UbSYRAP_pDONR201 was a gift from Francesco Licausi (Addgene plasmid # 249816 ; http://n2t.net/addgene:249816 ; RRID:Addgene_249816)

  • For your References section:

    A synthetic ERFVII-dependent circuit in yeast sheds light on the regulation of early hypoxic responses of plants. Lavilla-Puerta M, He Y, Piccinini L, Di Paco L, Papachristodoulou A, Licausi F, Giuntoli B. bioRxiv 2025.09.01.673502 10.1101/2025.09.01.673502
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