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pTA-M+
(Plasmid #249818)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249818 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pKW-08
  • Backbone manufacturer
    Addgene 25012
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 9000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Mycobacterium tuberculosis topoisomerase I
  • Alt name
    Mtb TopA
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    2802
  • GenBank ID
    NC_000962.3
  • Entrez Gene
    topA (a.k.a. Rv3646c)
  • Promoter Tet
  • Tag / Fusion Protein
    • 6xHis (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site ScaI (not destroyed)
  • 5′ sequencing primer pKSBluescript Universal primer TCGAGGTCGACGGTATC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTA-M+ was a gift from Yuk-Ching Tse-Dinh (Addgene plasmid # 249818 ; http://n2t.net/addgene:249818 ; RRID:Addgene_249818)

  • For your References section:

    Small-Molecule Inhibitors Targeting Topoisomerase I as Novel Antituberculosis Agents. Sandhaus S, Annamalai T, Welmaker G, Houghten RA, Paz C, Garcia PK, Andres A, Narula G, Rodrigues Felix C, Geden S, Netherton M, Gupta R, Rohde KH, Giulianotti MA, Tse-Dinh YC. Antimicrob Agents Chemother. 2016 Jun 20;60(7):4028-36. doi: 10.1128/AAC.00288-16. Print 2016 Jul. 10.1128/AAC.00288-16 PubMed 27114277
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