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pTA-noI
(Plasmid #249819)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 249819 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pKW-08
  • Backbone manufacturer
    Addgene 25012
  • Backbone size (bp) 6000
  • Modifications to backbone
    pKW-08 was religated following the removal of the luxAB genes by BamH1/ScaI digestion. The isolated plasmid has a deletion of 365 bp upstream of BamH1 site.
  • Vector type
    Bacterial Expression
  • Promoter Tet
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Hygromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer pBluescript KS Universal Primer TCGAGGTCGACGGTATC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTA-noI was a gift from Yuk-Ching Tse-Dinh (Addgene plasmid # 249819 ; http://n2t.net/addgene:249819 ; RRID:Addgene_249819)

  • For your References section:

    Small-Molecule Inhibitors Targeting Topoisomerase I as Novel Antituberculosis Agents. Sandhaus S, Annamalai T, Welmaker G, Houghten RA, Paz C, Garcia PK, Andres A, Narula G, Rodrigues Felix C, Geden S, Netherton M, Gupta R, Rohde KH, Giulianotti MA, Tse-Dinh YC. Antimicrob Agents Chemother. 2016 Jun 20;60(7):4028-36. doi: 10.1128/AAC.00288-16. Print 2016 Jul. 10.1128/AAC.00288-16 PubMed 27114277
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