pLIC-MTOP
(Plasmid
#250060)
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PurposeExpresses Mycobacterium tuberculosis topoisomerase I in E. coli under the control of the T7 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250060 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepLIC-HK
- Backbone size w/o insert (bp) 5486
- Total vector size (bp) 8285
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructions1% glucose
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMycobacterium tuberculosis topoisomerase I
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Alt nameMtb TopA
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SpeciesMycobacterium tuberculosis
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Insert Size (bp)2799
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GenBank IDNC_015848.1
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on backbone)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLIC-MTOP was a gift from Yuk-Ching Tse-Dinh (Addgene plasmid # 250060 ; http://n2t.net/addgene:250060 ; RRID:Addgene_250060) -
For your References section:
Analysis of DNA relaxation and cleavage activities of recombinant Mycobacterium tuberculosis DNA topoisomerase I from a new expression and purification protocol. Annamalai T, Dani N, Cheng B, Tse-Dinh YC. BMC Biochem. 2009 Jun 11;10:18. doi: 10.1186/1471-2091-10-18. 10.1186/1471-2091-10-18 PubMed 19519900
