Skip to main content
Addgene
Open Menu

pGEX-SMG7-14-3-3
(Plasmid #250069)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250069 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pGEX-4T-1
  • Backbone size w/o insert (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMG7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    762
  • Mutation
    None
  • Entrez Gene
    SMG7 (a.k.a. C1orf16, EST1C, SGA56M)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NCBI identifier: NP_963862

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-SMG7-14-3-3 was a gift from Mark Bedford (Addgene plasmid # 250069 ; http://n2t.net/addgene:250069 ; RRID:Addgene_250069)

  • For your References section:

    PRMT5 C-terminal Phosphorylation Modulates a 14-3-3/PDZ Interaction Switch. Espejo AB, Gao G, Black K, Gayatri S, Veland N, Kim J, Chen T, Sudol M, Walker C, Bedford MT. J Biol Chem. 2017 Feb 10;292(6):2255-2265. doi: 10.1074/jbc.M116.760330. Epub 2016 Dec 28. 10.1074/jbc.M116.760330 PubMed 28031468
Related items: