pGEX-gamma2-syntrophin-PDZ-1
(Plasmid
#250073)
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PurposeBacterial expression of gamma2-syntrophin PDZ domain 1
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepGEX-4T-1
- Backbone size w/o insert (bp) 4900
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namegamma2-syntrophin (1/1)
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SpeciesM. musculus (mouse)
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Insert Size (bp)264
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MutationNone
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Entrez GeneSntg2 (a.k.a. BB121248, 9530013L23, 2210008K22Rik)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
UniProt ID: Q925E0
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX-gamma2-syntrophin-PDZ-1 was a gift from Mark Bedford (Addgene plasmid # 250073 ; http://n2t.net/addgene:250073 ; RRID:Addgene_250073) -
For your References section:
PRMT5 C-terminal Phosphorylation Modulates a 14-3-3/PDZ Interaction Switch. Espejo AB, Gao G, Black K, Gayatri S, Veland N, Kim J, Chen T, Sudol M, Walker C, Bedford MT. J Biol Chem. 2017 Feb 10;292(6):2255-2265. doi: 10.1074/jbc.M116.760330. Epub 2016 Dec 28. 10.1074/jbc.M116.760330 PubMed 28031468