pAAV Syn-CD4-CIBN-V5-TurboID-NES
(Plasmid
#250173)
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PurposeMembrane-TurboID variant for neuron expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backboneAAV
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCD4
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SpeciesH. sapiens (human)
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Entrez GeneCD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
- Promoter hSyn
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Tag
/ Fusion Protein
- CIBN-V5-TurboID-NES (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer actcagcgctgcctcagtctgc
- (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV Syn-CD4-CIBN-V5-TurboID-NES was a gift from Christina Kim (Addgene plasmid # 250173 ; http://n2t.net/addgene:250173 ; RRID:Addgene_250173)