Skip to main content
Addgene
Open Menu

pWZLhygro-MyoD-K/R-ER(T)-3xT7
(Plasmid #250216)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250216 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    pWZLhygro
  • Backbone size w/o insert (bp) 5915
  • Total vector size (bp) 7975
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    MyoD-K/R-ER(T)-3xT7
  • Alt name
    MyoD
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2060
  • Mutation
    changed lysines (K) 99, K102, and K104 to arginines (R)
  • Entrez Gene
    Myod1 (a.k.a. MYF3, MyoD, Myod-1, bHLHc1)
  • Tag / Fusion Protein
    • 3xT7 tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pBABE 5'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWZLhygro-MyoD-K/R-ER(T)-3xT7 was a gift from Kai Ge (Addgene plasmid # 250216 ; http://n2t.net/addgene:250216 ; RRID:Addgene_250216)
Related items: