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Protein2PAM_NmeN4C.3
(Plasmid #250220)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250220 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    n/a
  • Backbone manufacturer
    Twist
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    NmeN4C.3
  • Species
    Synthetic
  • Mutation
    Nme1Cas9 E954C/D957G/Q981G/F1020Y/A1021S/C1023L/H1024D/G1026S/N1031S/L1037K/I1046V/E1048R
  • Promoter CMV
  • Tag / Fusion Protein
    • NLS-3xFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Protein2PAM guided computational evolution towards a N4C PAM. Please visit https://doi.org/10.1101/2025.01.06.631536 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Protein2PAM_NmeN4C.3 was a gift from Ali Madani (Addgene plasmid # 250220 ; http://n2t.net/addgene:250220 ; RRID:Addgene_250220)
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