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pCMV-SNAPtag-PixE
(Plasmid #250232)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250232 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3968
  • Total vector size (bp) 5741
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PixE
  • Alt name
    Slr1693
  • Species
    Synthetic
  • Entrez Gene
    slr1693 (a.k.a. SYNGTS_1813)
  • Promoter CMV
  • Tag / Fusion Protein
    • SNAPtag (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGGAGGGGGAGGCTccggaatgagcaattcagttttg
  • 3′ sequencing primer GTTATCTAGATCCGGTGGATCctcaggagttggttttattg
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Additional sequencing primer may be needed: gcattaatgctacagcccat

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-SNAPtag-PixE was a gift from Won Do Heo (Addgene plasmid # 250232 ; http://n2t.net/addgene:250232 ; RRID:Addgene_250232)

  • For your References section:

    Optogenetic storage and release of protein and mRNA in live cells and animals. Lee C, Yu J, Shin J, Yu J, Heo Y, Lee M, Yu D, Park Y, Heo WD. Nat Commun. 2025 Jul 7;16(1):6230. doi: 10.1038/s41467-025-61322-y. 10.1038/s41467-025-61322-y PubMed 40624072
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