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pJC2527 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-StrepII-FBXO31(∆1-65)
(Plasmid #250252)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250252 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    SKP1
  • Species
    H. sapiens (human)
  • Mutation
    A2P, ∆38-43, ∆71-82
  • Entrez Gene
    SKP1 (a.k.a. EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A)
  • Promoter T7

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    FBXO31
  • Species
    H. sapiens (human)
  • Mutation
    ∆1-65
  • Entrez Gene
    FBXO31 (a.k.a. FBX14, FBXO14, Fbx31, MRT45, pp2386)
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis-Smt3(SUMO)-StrepII (N terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJC2527 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-StrepII-FBXO31(∆1-65) was a gift from Jacob Corn (Addgene plasmid # 250252 ; http://n2t.net/addgene:250252 ; RRID:Addgene_250252)

  • For your References section:

    C-terminal amides mark proteins for degradation via SCF-FBXO31. Muhar MF, Farnung J, Cernakova M, Hofmann R, Henneberg LT, Pfleiderer MM, Denoth-Lippuner A, Kalcic F, Nievergelt AS, Peters Al-Bayati M, Sidiropoulos ND, Beier V, Mann M, Jessberger S, Jinek M, Schulman BA, Bode JW, Corn JE. Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w. 10.1038/s41586-024-08475-w PubMed 39880951
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