pJC2747 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-FBXO31(∆1-65, D334N)
(Plasmid #250278)

• Purpose: Recombinant production of SKP1(A2P, ∆38-43, ∆71-82) and 6His-Smt3-StrepII-FBXO31(∆1-65, D334N).
• Depositing Lab: Jacob Corn
• Publication: Muhar et al Nature. 2025 Feb;638(8050):519-527. doi: 10.1038/s41586-024-08475-w.

Backbone

• Vector backbone: pET28
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: SKP1
• Species: H. sapiens (human)
• Mutation: A2P, ∆38-43, ∆71-82
• Entrez Gene: SKP1 (a.k.a. EMC19, OCP-II, OCP2, SKP1A, TCEB1L, p19A)
• Promoter: T7

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Gene/Insert 2

• Gene/Insert name: FBXO31
• Species: H. sapiens (human)
• Mutation: ∆1-65, D334N
• Entrez Gene: FBXO31 (a.k.a. FBX14, FBXO14, Fbx31, MRT45, pp2386)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-Smt3(SUMO)-StrepII (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Supplemental Documents:
  • pJC2747 - pET28-SKP1(A2P, ∆38-43, ∆71-82)-StrepII-FBXO31(Δ1-65, D334N).gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry