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pBdox-SPI1-T2A-CEBPA-P2A-FLI1_BleoR
(Plasmid #250291)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250291 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBdox
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SPI1-T2A-CEBPA-P2A-FLI1
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBdox-SPI1-T2A-CEBPA-P2A-FLI1_BleoR was a gift from George Church (Addgene plasmid # 250291 ; http://n2t.net/addgene:250291 ; RRID:Addgene_250291)

  • For your References section:

    Iterative transcription factor screening enables rapid generation of microglia-like cells from human iPSC. Liu S, Li L, Zhang F, Garcia-Corral M, Meyer K, Fortuna PRJ, van Sambeek B, Appleton E, Ng AHM, Khoshakhlagh P, Lu YR, Cameron J, Ramirez RN, Chen Y, Wu CT, Huang JY, Tan Y, Chao G, Aach J, Lim ET, Tam JM, Raychaudhuri S, Church GM. Nat Commun. 2025 Jun 10;16(1):5136. doi: 10.1038/s41467-025-59596-3. 10.1038/s41467-025-59596-3 PubMed 40494892
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