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pINEAPLE
(Plasmid #250295)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250295 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    Custom
  • Backbone manufacturer
    Maurano lab, The Center for Synthetic Regulatory Genomics, NYU Langone Health
  • Backbone size w/o insert (bp) 4858
  • Total vector size (bp) 5916
  • Vector type
    Synthetic Biology ; assembly vector for Big-IN payloads
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Grows as green/white colonies
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SuperfolderGFP
  • Insert Size (bp)
    1058

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.08.13.669251 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pINEAPLE was a gift from Matthew Maurano (Addgene plasmid # 250295 ; http://n2t.net/addgene:250295 ; RRID:Addgene_250295)

  • For your References section:

    Synthetic genomic dissection of enhancer context sensitivity and synergy. Ordoñez R, Ribeiro-dos-Santos AM, McLoughlin C, Ellis G, Ashe HJ, Berastegui Zufiaurre N, Brosh R, Majewski M, Camellato B, Boeke JD, Maurano MT. bioRxiv 2025.08.13.669251 10.1101/2025.08.13.669251
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