pLVX-FUT3-IRES-puro
(Plasmid #250317)

• Purpose: Expression of human FUT3 with C-terminal FLAG tag in mammalian cells
• Depositing Lab: Jennifer Kohler
• Publication: Konada et al Glycobiology. 2026 Feb 26:cwag011. doi: 10.1093/glycob/cwag011.

Backbone

• Vector backbone: pLVX-IRES-puro
• Backbone manufacturer: Takara Bio cat# 632183
• Backbone size w/o insert (bp): 8140
• Total vector size (bp): 9178
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FUT3
• Species: H. sapiens (human)
• Insert Size (bp): 1083
• Mutation: synonymous mutations to DNA encoding Ser253 and Arg254
• Entrez Gene: FUT3 (a.k.a. CD174, FT3B, FucT-III, LE, Les)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Invitrogen Ultimate ORF clones library

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry