pcDNA3.1-hVEGFR3-mTurquoise
(Plasmid
#250335)
-
PurposeExpression of the human VEGFR3 tagged with C-terminal mTurquoise tag
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250335 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepcDNA3.1-V5/His
-
Backbone manufacturerInvitrogen
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namehuman VEGFR3, full length
-
SpeciesH. sapiens (human)
-
Entrez GeneFLT4 (a.k.a. CHTD7, FLT-4, FLT41, LMPH1A, LMPHM1, PCL, VEGFR-3, VEGFR3)
- Promoter CMV
-
Tag
/ Fusion Protein
- mTurquoise (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unspecified (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A T28P mutation was found in VEGFR3 that does not affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pcDNA3.1-hVEGFR3-mTurquoise was a gift from Pavel Krejčí (Addgene plasmid # 250335 ; http://n2t.net/addgene:250335 ; RRID:Addgene_250335)