pCAG-ATG101ΔCTH
(Plasmid
#250340)
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PurposeExpression human ATG101ΔCTH
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 250340 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepCAG
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameATG101
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Alt nameC12orf44
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SpeciesH. sapiens (human)
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Insert Size (bp)594
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GenBank IDNP_068753.2
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Entrez GeneATG101 (a.k.a. C12orf44)
- Promoter CAG
Cloning Information
- Cloning method Gene Synthesis
- 5′ sequencing primer GTTCGGCTTCTGGCGTGT
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.11.07.687251 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-ATG101ΔCTH was a gift from James Hurley (Addgene plasmid # 250340 ; http://n2t.net/addgene:250340 ; RRID:Addgene_250340) -
For your References section:
Reconstitution of multistep recruitment of ULK1 to membranes in autophagy. Duan Y, Lu Y, Paul S, Betz J, Wilhelm L, Cook ASI, Ren X, Adriaenssens E, Martens S, Ganley IG, Hummer G, Hurley JH. bioRxiv [Preprint]. 2025 Nov 9:2025.11.07.687251. doi: 10.1101/2025.11.07.687251. 10.1101/2025.11.07.687251 PubMed 41278708