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p2OT-MtbTOP1
(Plasmid #250351)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250351 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    2O-T
  • Backbone manufacturer
    Scott Gradia
  • Backbone size w/o insert (bp) 5155
  • Total vector size (bp) 7957
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    1% glucose
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Mycobacterium tuberculosis topoisomerase I
  • Alt name
    Mtb TopA
  • Species
    Mycobacterium tuberculosis
  • Insert Size (bp)
    2802
  • GenBank ID
    NC_015848.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis mocr (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p2OT-MtbTOP1 was a gift from Yuk-Ching Tse-Dinh (Addgene plasmid # 250351 ; http://n2t.net/addgene:250351 ; RRID:Addgene_250351)

  • For your References section:

    Insights from the Structure of Mycobacterium tuberculosis Topoisomerase I with a Novel Protein Fold. Tan K, Cao N, Cheng B, Joachimiak A, Tse-Dinh YC. J Mol Biol. 2016 Jan 16;428(1):182-193. doi: 10.1016/j.jmb.2015.11.024. Epub 2015 Dec 3. 10.1016/j.jmb.2015.11.024 PubMed 26655023
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