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pET22b_Hsp10
(Plasmid #250361)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250361 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET22b
  • Backbone size w/o insert (bp) 5365
  • Total vector size (bp) 5672
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    10 kDa heat shock protein, mitochondrial
  • Alt name
    Hsp10
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    306
  • GenBank ID
    3336
  • Entrez Gene
    HSPE1 (a.k.a. CPN10, EPF, GROES, HSP10)
  • Promoter T7

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer atggcaggacaagcgtt
  • 3′ sequencing primer tcttggaaagtacgtagac
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    C. Weiss, PhD.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET22b_Hsp10 was a gift from Abdussalam Azem (Addgene plasmid # 250361 ; http://n2t.net/addgene:250361 ; RRID:Addgene_250361)
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