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pET21D_HspD1
(Plasmid #250362)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 250362 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET21D
  • Backbone size w/o insert (bp) 5382
  • Total vector size (bp) 7098
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    60 kDa heat shock protein, mitochondrial
  • Alt name
    Hsp60
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1716
  • GenBank ID
    3329
  • Entrez Gene
    HSPD1 (a.k.a. CPN60, GROEL, HLD4, HSP-60, HSP60, HSP65, HuCHA60, SPG13)
  • Promoter T7
  • Tag / Fusion Protein
    • 8xHis and TEV site (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer tgcaccaaattttacatctttggc
  • 3′ sequencing primer gaacatgccacctcccataccacctc
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    C. Weiss, PhD.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21D_HspD1 was a gift from Abdussalam Azem (Addgene plasmid # 250362 ; http://n2t.net/addgene:250362 ; RRID:Addgene_250362)
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